Extracurricular laboratory: Synthetic route of 1H-1,2,4-Triazol-5-amine

Application In Synthesis of 1H-1,2,4-Triazol-5-amine. Welcome to talk about 61-82-5, If you have any questions, you can contact Sweidan, NI; El-Abadelah, MM; Nazer, MZ; Voelter, W or send Email.

Sweidan, NI; El-Abadelah, MM; Nazer, MZ; Voelter, W in [Voelter, Wolfgang] Univ Tubingen, Interfak Inst Biochem, Hoppe Seyler Str 4, D-72076 Tubingen, Germany; [Sweidan, Nuha, I] Univ Petra, Fac Art & Sci, Dept Chem, Amman 961343, Jordan; [El-Abadelah, Mustafa M.; Nazer, Musa Z.] Univ Jordan, Fac Sci, Dept Chem, Amman 11942, Jordan published Selective cyclization modes of methyl 3 ‘-heteroarylamino-2 ‘-(2,5-dichlorothiophene-3-carbonyl)acrylates. Synthesis of model (thienyl) pyrazolo- and triazolo[1,5-alpha]pyrimidines in 2020.0, Cited 23.0. Application In Synthesis of 1H-1,2,4-Triazol-5-amine. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5.

Interaction of methyl 3-ethoxy-2-(2,5-dichloro-3-thenoyl)acrylate (I) with 3-aminopyrazole and 3- amino-1,2,4-triazole generated the respective pyrazolo [1,5-alpha]pyrimidine (4) and triazolo[1,5-alpha]pyrimidine (7). The formation of 4 entails selective and consecutive displacement of the 3-ethoxy and methoxy (ester) anions in I by 3-NH2 and 1-NH of 3-aminopyrazole. On the other hand, the formation of 7 implies selective displacement of 3-ethoxy in I by the ring-NH followed by cyclocondensation involving the keto group in I and 3-NH2 of aminotriazole. This latter selective displacement sequence is also followed by 3-amino-5-hydroxypyrazole in its reaction with I. The structures of the new compounds are supported by microanalytical and spectral data.

Application In Synthesis of 1H-1,2,4-Triazol-5-amine. Welcome to talk about 61-82-5, If you have any questions, you can contact Sweidan, NI; El-Abadelah, MM; Nazer, MZ; Voelter, W or send Email.

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

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Welcome to talk about 61-82-5, If you have any questions, you can contact Silva, EN; Silveira, JAG; Aragao, RM; Vieira, CF; Carvalho, FEL or send Email.. SDS of cas: 61-82-5

In 2019.0 ACTA PHYSIOL PLANT published article about HYDROGEN-PEROXIDE; WATER-STRESS; MESOPHYLL CONDUCTANCE; ASCORBATE PEROXIDASE; OSMOTIC ADJUSTMENT; PLANTS; MECHANISMS; LEAVES; ACCLIMATION; SALINITY in [Silva, Evandro Nascimento] Univ Estadual Ceara, Fac Educ Ciencias & Letras Sertao Cent, BR-63900000 Quixada, Ceara, Brazil; [Silveira, Joaquim A. G.; Vieira, Cinthya F.; Carvalho, Fabricio E. L.] Univ Fed Ceara, Dept Bioquim & Biol Mol, Lab Metab Plantas, Campus Pici,CP 6020, BR-60451970 Fortaleza, Ceara, Brazil; [Aragao, Rafael M.] UFRA, Campus Capanema, BR-68700030 Capanema, Para, Brazil in 2019.0, Cited 58.0. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5. SDS of cas: 61-82-5

Catalase (CAT) is a crucial enzyme to control the excess peroxisomal H2O2 produced during photorespiration. In many plant species, this enzymatic activity decreases in response to drought but its specific role in photosynthesis and redox metabolism is still poorly understood. In this study was tested the hypothesis that photosynthetic and oxidative changes induced by drought are dependent on CAT activity. For this, Jatropha curcas, a drought-tolerant species, was subjected to water deficit and CAT inhibition by a specific pharmacological inhibitor (3-AT), in order to decrease the activity of this enzyme to a similar level as compared to that exhibited by water deficit-treated plants. The CO2 assimilation and other photosynthetic-related parameters were decreased more intensively by drought as compared to plants exposed to 3-AT, whereas the photochemical efficiency of PSII remained unchanged in both conditions. Non-photochemical quenching was strongly increased in drought-treated plants, but only slightly increased in 3-AT treatment. Membrane integrity and lipid peroxidation were strongly increased in both treatments, while H2O2 content was increased only by drought imposition. Ascorbate peroxidase and superoxide dismutase activities were increased in both drought and 3-AT treatments, but glycolate oxidase was strongly increased only in drought-stressed plants. The obtained results evidence that CO2 assimilation and oxidative protection in leaves of Jatropha curcas plants exposed to water deficit are greatly dependent on drought-induced CAT activity deficiency.

Welcome to talk about 61-82-5, If you have any questions, you can contact Silva, EN; Silveira, JAG; Aragao, RM; Vieira, CF; Carvalho, FEL or send Email.. SDS of cas: 61-82-5

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

Archives for Chemistry Experiments of 61-82-5

About 1H-1,2,4-Triazol-5-amine, If you have any questions, you can contact Kassem, AF; Abbas, EMH; Al-Qurashi, NT; Farghaly, TA or concate me.. Computed Properties of C2H4N4

An article New azoloazine derivatives as antimicrobial agents: Synthesis under microwave irradiations, structure elucidation, and antimicrobial activity WOS:000503541800001 published article about ONE-POT SYNTHESIS; ASSISTED SYNTHESIS; REARRANGEMENT; HETEROCYCLES in [Kassem, Asmaa F.; Abbas, Eman M. H.] Natl Res Ctr, Pharmaceut & Drug Ind Res Div, Chem Nat & Microbial Prod Dept, 33 Bohouth St,POB 12622, Giza, Egypt; [Al-Qurashi, Nadia T.] Umm Al Qura Univ, Univ Coll Adam, Dept Basic Sci, Mecca, Almukkarramah, Saudi Arabia; [Farghaly, Thoraya A.] Cairo Univ, Fac Sci, Dept Chem, Giza 1263, Egypt; [Farghaly, Thoraya A.] Umm Al Qura Univ, Fac Appl Sci, Dept Chem, Mecca 21514, Almukkarramah, Saudi Arabia in 2020.0, Cited 28.0. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5. Computed Properties of C2H4N4

With aiding of microwave radiation, new series of fused-thiazolopyrimidines and fused-triazolopyrimidines each with cycloheptane have been synthesized through the reaction of cycloheptane-thione with alpha-haloketones or hydrazonoyl chlorides in short-time intervals as well as high yield. Moreover, reaction of 2,7-bis(2-chlorobenzylidene)cycloheptan-1-one with o-aminothiophenol and heterocyclic amines afforded benzo[b]cyclohepta[e][1,4]thiazepine derivative and cycloheptapyrimidines fused with different azoles, respectively. The structure for all products was discussed and proved based on the results of spectral data and elemental analysis. Evaluation of the antimicrobial activity of selected products indicated that most of derivatives showed their potent activity exceeds the reference antibiotic in some cases.

About 1H-1,2,4-Triazol-5-amine, If you have any questions, you can contact Kassem, AF; Abbas, EMH; Al-Qurashi, NT; Farghaly, TA or concate me.. Computed Properties of C2H4N4

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

Can You Really Do Chemisty Experiments About 1H-1,2,4-Triazol-5-amine

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An article Synthesis, biological evaluation and DFT calculation of novel pyrazole and pyrimidine derivatives WOS:000456491500033 published article about DENSITY-FUNCTIONAL THEORY; ANTIMICROBIAL EVALUATION; REGIOSELECTIVE SYNTHESIS; VERSATILE PRECURSORS; MOLECULAR-STRUCTURE; CRYSTAL-STRUCTURE; INHIBITORS; DOCKING; OPTIMIZATION; HETEROCYCLES in [Farag, Ahmad M.] Cairo Univ, Dept Chem, Fac Sci, Giza 12613, Egypt; [Fahim, Asmaa M.] Natl Res Ctr Dokki, Dept Green Chem, POB 12622, Cairo, Egypt in 2019.0, Cited 66.0. Name: 1H-1,2,4-Triazol-5-amine. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5

The utility of the enaminonitriles 3a and 3b for the synthesis of the pyrazole derivatives 5a,b, 7a,b, diaminopyrimidine derivatives 9,11, pyrazolo[1,5-alpha]pyrimidines 12,15, triazolo[4,3-alpha]pyrimidines 13,16, imidazo[1,2-alpha]pyrimidine derivatives 14 and 17, was explored. Most of the synthesized compounds showed excellent in vitro antitumor activity against MCF-7 cell line. They also exhibted high antimicrobial and antioxidant activities. Density functional theory (DFT) calculations at the B3LYP/6-31G level of theory have been carried out to investigate the equilibrium geometry of the novel phenylpyrazolo[1,5-alpha]pyrimidin-6-yl)methanone derivative 12 and phenylpyrazolo[1,5-alpha]pyrimidine derivative 15. The structure-activity relationship (SAR) has been used to correlate the biological activity with the appropriate quantum such as total energy. The energy of the HOMO and LUMO and Mulliken atomic charges were also calculated. (C) 2018 Elsevier B.V. All rights reserved.

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Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

Chemistry Milestones Of 1H-1,2,4-Triazol-5-amine

Welcome to talk about 61-82-5, If you have any questions, you can contact Sipos, D; Laszlo, Z; Toth, Z; Kovacs, P; Tollar, J; Gulyban, A; Lakosi, F; Repa, I; Kovacs, A or send Email.. Computed Properties of C2H4N4

I found the field of Oncology very interesting. Saw the article Additional Value of 18F-FDOPA Amino Acid Analog Radiotracer to Irradiation Planning Process of Patients With Glioblastoma Multiforme published in 2021.0. Computed Properties of C2H4N4, Reprint Addresses Sipos, D (corresponding author), Moritz Kaposi Teaching Hosp, Dr Jozsef Bake Diagnost Radiat Oncol Res & Teachi, Kaposvar, Hungary.; Sipos, D (corresponding author), Univ Pecs, Doctoral Sch Hlth Sci, Pecs, Hungary.; Sipos, D (corresponding author), Univ Pecs, Dept Med Imaging, Fac Hlth Sci, Pecs, Hungary.. The CAS is 61-82-5. Through research, I have a further understanding and discovery of 1H-1,2,4-Triazol-5-amine

Purpose To investigate the added value of 6-(18F]-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) PET to radiotherapy planning in glioblastoma multiforme (GBM). Methods From September 2017 to December 2020, 17 patients with GBM received external beam radiotherapy up to 60 Gy with concurrent and adjuvant temozolamide. Target volume delineations followed the European guideline with a 2-cm safety margin clinical target volume (CTV) around the contrast-enhanced lesion+resection cavity on MRI gross tumor volume (GTV). All patients had FDOPA hybrid PET/MRI followed by PET/CT before radiotherapy planning. PET segmentation followed international recommendation: T/N 1.7 (BTV1.7) and T/N 2 (BTV2.0) SUV thresholds were used for biological target volume (BTV) delineation. For GTV-BTVs agreements, 95% of the Hausdorff distance (HD95%) from GTV to the BTVs were calculated, additionally, BTV portions outside of the GTV and coverage by the 95% isodose contours were also determined. In case of recurrence, the latest MR images were co-registered to planning CT to evaluate its location relative to BTVs and 95% isodose contours. Results Average (range) GTV, BTV1.7, and BTV2.0 were 46.58 (6-182.5), 68.68 (9.6-204.1), 42.89 (3.8-147.6) cm(3), respectively. HD95% from GTV were 15.5 mm (7.9-30.7 mm) and 10.5 mm (4.3-21.4 mm) for BTV1.7 and BTV2.0, respectively. Based on volumetric assessment, 58.8% (28-100%) of BTV1.7 and 45.7% of BTV2.0 (14-100%) were outside of the standard GTV, still all BTVs were encompassed by the 95% dose. All recurrences were confirmed by follow-up imaging, all occurred within PTV, with an additional outfield recurrence in a single case, which was not DOPA-positive at the beginning of treatment. Good correlation was found between the mean and median values of PET/CT and PET/MRI segmented volumes relative to corresponding brain-accumulated enhancement (r = 0.75; r = 0.72). Conclusion (18F)FDOPA PET resulted in substantial larger tumor volumes compared to MRI; however, its added value is unclear as vast majority of recurrences occurred within the prescribed dose level. Use of PET/CT signals proved to be feasible in the absence of direct segmentation possibilities of PET/MR in TPS. The added value of (18F)FDOPA may be better exploited in the context of integrated dose escalation.

Welcome to talk about 61-82-5, If you have any questions, you can contact Sipos, D; Laszlo, Z; Toth, Z; Kovacs, P; Tollar, J; Gulyban, A; Lakosi, F; Repa, I; Kovacs, A or send Email.. Computed Properties of C2H4N4

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

Why Are Children Getting Addicted To 1H-1,2,4-Triazol-5-amine

SDS of cas: 61-82-5. Welcome to talk about 61-82-5, If you have any questions, you can contact Yamaguchi, K; Yoshimura, Y; Nakagawa, S; Mezaki, H; Yoshimura, S; Kawasaki, T or send Email.

SDS of cas: 61-82-5. In 2019.0 BIOSCI BIOTECH BIOCH published article about NADPH OXIDASE RBOHD; KINASE BIK1; RICE; RECEPTOR; PROTEIN; BIOGENESIS; EFFECTOR; CLUSTERS; CEBIP; DRE2 in [Yamaguchi, Koji; Yoshimura, Yuya; Nakagawa, Shinya; Mezaki, Hirokazu; Yoshimura, Satomi; Kawasaki, Tsutomu] Kindai Univ, Grad Sch Agr, Dept Adv Biosci, Naka, Nara, Japan in 2019.0, Cited 24.0. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5.

The rice receptor-like cytoplasmic kinase 185 (OsRLCK185) interacts with the chitin receptor complex OsCERK1/CEBiP and positively regulates chitin-induced immune responses including MAP kinase activation, ROS production and defense gene expression. To elucidate the regulatory mechanisms of OsRLCK185-mediated immunity, we searched for interactors of OsRLCK185. OsDRE2a, rice homologs of the yeast Dre2 protein, were identified as novel interactors of OsRLCK185. OsDRE2a interacted with OsRLCK185 at plasma membrane. The conserved cysteine residues in CIAPIN1 domain of OsDRE2a were essential for tight interaction of OsRLCK185. OsDRE2a was phosphorylated by OsRLCK185. The expression of OsDRE2a and OsDRE2b was induced after chitin treatment. Reduction of OsDRE2a and OsDRE2b mRNA levels by RNA interference resulted in the decreased chitin-induced ROS production. Thus, it is likely that OsDRE2 regulates OsRLCK185-mediated immune responses.

SDS of cas: 61-82-5. Welcome to talk about 61-82-5, If you have any questions, you can contact Yamaguchi, K; Yoshimura, Y; Nakagawa, S; Mezaki, H; Yoshimura, S; Kawasaki, T or send Email.

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

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Product Details of 61-82-5. In 2020.0 MOLECULES published article about HETEROCYCLES; DERIVATIVES; DESIGN in [El Azab, Islam H.] Taif Univ, Fac Sci, Chem Dept, POB 888, At Taif 2974, Saudi Arabia; [Elkanzi, Nadia A. A.] Jouf Univ, Coll Sci, Chem Dept, POB 2014, Sakaka, Saudi Arabia in 2020.0, Cited 31.0. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5.

In search of unprecedented tri and/or tetrapod pharmacophoric conjugates, a series of 32 new 4-ethyl-1H-benzo[b][1,4]diazepin-2(3H)-ones were synthesized and properly elucidated using MS, IR, NMR, and elemental analysis. In vitro investigation of 11 compounds of this series, using a panel of two human tumor cell lines namely; human breast adenocarcinoma (MCF-7), and human colorectal carcinoma (HCT-116), revealed promising cytotoxic activities. Among all synthesized compounds, analogue 9 displayed maximum cytotoxicity with IC50 values of 16.19 +/- 1.35 and 17.16 +/- 1.54 mu M against HCT-116 and MCF-7, respectively, compared to standard drug doxorubicin.

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Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

Extended knowledge of 61-82-5

About 1H-1,2,4-Triazol-5-amine, If you have any questions, you can contact Galimberti, F; Moretto, A; Papa, E or concate me.. HPLC of Formula: C2H4N4

Authors Galimberti, F; Moretto, A; Papa, E in PERGAMON-ELSEVIER SCIENCE LTD published article about DIFFERENT VALIDATION CRITERIA; REAL EXTERNAL PREDICTIVITY; CORRELATION-COEFFICIENT; IDENTIFICATION; ALTERNATIVES; EVALUATE; HAZARD in [Galimberti, Francesco; Moretto, Angelo] ASST Fatebenefratelli Sacco, Int Ctr Pesticides & Hlth Risk Prevent, ICPS, Milan, Italy; [Moretto, Angelo] Univ Milan, Dept Biomed & Clin Sci, Milan, Italy; [Papa, Ester] Univ Insubria, QSAR Res Unit Environm Chem & Ecotoxicol, Varese, Italy in 2020.0, Cited 45.0. HPLC of Formula: C2H4N4. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5

The EFSA ‘Guidance on tiered risk assessment for edge-of-field surface waters’ underscores the importance of in silico models to support the pesticide risk assessment. The aim of this work was to use in silico models starting from an available, structured and harmonized pesticide dataset that was developed for different purposes, in order to stimulate the use of QSAR models for risk assessment. The present work focuses on the development of a set of in silico models, developed to predict the aquatic toxicity of heterogeneous pesticides with incomplete/unknown toxic behavior in the water compartment. The generated models have good fitting performances (R-2 : 0.75-0.99), they are internally robust (Q(2) loo: 0.66-0.98) and can handle up to 30% of perturbation of the training set (Q(2) Imo: 0.64-0.98). The absence of chance correlation was guaranteed by low values of R-2 calculated on scrambled responses (R-2 Y-scr: 0.11-0.38). Different statistical parameters were used to quantify the external predictivity of the models (CCCext: 0.73-0.91, Q(2) ext-Fn: 0.53-0.96). The results indicate that all the best models are predictive when applied to chemicals not involved in the models development. In addition, all models have similar accuracy both in fitting and in prediction and this represents a good degree of generalization. These models may be useful to support the risk assessment procedure when experimental data for key species are missing or to create prioritization lists for the general a priori assessment of the potential toxicity of existing and new pesticides which fall in the applicability domain. (C) 2020 Elsevier Ltd. All rights reserved.

About 1H-1,2,4-Triazol-5-amine, If you have any questions, you can contact Galimberti, F; Moretto, A; Papa, E or concate me.. HPLC of Formula: C2H4N4

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

Archives for Chemistry Experiments of 1H-1,2,4-Triazol-5-amine

Product Details of 61-82-5. Welcome to talk about 61-82-5, If you have any questions, you can contact Abdel-Kader, NS; Moustafa, H; El-Ansary, AL; Sherif, OE; Farghaly, AM or send Email.

Product Details of 61-82-5. Authors Abdel-Kader, NS; Moustafa, H; El-Ansary, AL; Sherif, OE; Farghaly, AM in ROYAL SOC CHEMISTRY published article about in [Abdel-Kader, Nora S.; Moustafa, Hussein; El-Ansary, Aida L.; Sherif, Omaima E.] Cairo Univ, Fac Sci, Chem Dept, Giza, Egypt; [Farghaly, Aya M.] Cairo Univ, Ctr Environm Hazard Mitigat, Giza, Egypt in 2021.0, Cited 71.0. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5

8-Acetyl-7-hydroxy-4-methyl coumarin and 3-amion-1,2,4-triazole were used to prepare a Schiff base (Sbat). The [Ag(Sbat)(NO3)]center dot H2O and [Cu(Sbat)(OH)(H2O)(2)]center dot 3H(2)O complexes were separated by the reaction of the Schiff base with Ag(i) and Cu(ii) metal ions. All the prepared compounds were subjected to elemental and spectral analyses (IR, UV-vis, H-1 NMR, and mass spectroscopy). Moreover, the complexes were also subjected to TG analysis, molar conductance measurements, magnetic moment analysis, and XRD measurements. The value of the molar conductance designates their non-electrolytic character. The local minimum structure, global properties and nonlinear optical parameters of the Schiff base were calculated at the B3LYP/6-31++(d,p) level of theory and those of its complexes were calculated at the B3LYP/GENECP level. The electronic spectra in the UV-visible region of the Schiff base (Sbat) and [Ag(Sbat)(NO3)]center dot H2O and [Cu(Sbat)(OH)(H2O)(2)]center dot 3H(2)O complexes in DMSO as a solvent were investigated experimentally and theoretically using the time dependent density functional theory (TD-DFT) method at the CAM-B3LYP/6-311++G(d,p) level of theory. The studied compounds were screened for their in vitro antibacterial activity against three Gram-positive (B. subtilis, S. aureus and S. faecalis) and three Gram-negative bacteria (E. coli, N. gonorrhoeae and P. auregenosa). An antifungal assay was performed against two fungal (C. albicans and A. flavus) species. The Ag-Sbat complex showed the highest antimicrobial activity. The cytotoxic activity of the Schiff base and its silver complex was tested in vitro against the MCF-7 (breast cancer) and HCT-116 (colon cancer) cell lines.

Product Details of 61-82-5. Welcome to talk about 61-82-5, If you have any questions, you can contact Abdel-Kader, NS; Moustafa, H; El-Ansary, AL; Sherif, OE; Farghaly, AM or send Email.

Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics

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An article Screening and analysis of agouti signaling protein interaction partners in Pelodiscus sinensis suggests a role in lipid metabolism WOS:000541109300072 published article about DIABETES-MELLITUS; ADIPOSE-TISSUES; EXPRESSION; RECEPTOR; OBESITY; MOUSE; IDENTIFICATION; ASIP; VITRONECTIN; FILAMINS in [Zhang, Lili; Si, Yue-Xiu] Zhejiang Chinese Med Univ, Sch Basic Med Sci, Hangzhou 310053, Peoples R China; [Zhang, Lili; Yin, Shang-Jun; Zheng, Xiaoying; Chen, Xuanwei; Wang, Qian; Park, Yong-Doo; Qian, Guo-Ying] Zhejiang Wanli Univ, Coll Biol & Environm Sci, Ningbo 315100, Peoples R China; [Park, Yong-Doo] Tsinghua Univ, Skin Dis Res Ctr, Yangtze Delta Reg Inst, Jiaxing 314006, Peoples R China; [Park, Yong-Doo] Sungkyunkwan Univ, Samsung Med Ctr, Sch Med, Dept Dermatol, Seoul 135710, South Korea in 2020.0, Cited 51.0. Recommanded Product: 61-82-5. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5

Agouti signaling protein (ASP) is a secreted paracrine protein that has been widely reported to function in melanogenesis and obesity and could potentially be a core protein that regulates the color and fatty phenotype of P. sinensis. In this study, we screened out interacting proteins of ASP by combined co-immunoprecipitation mass spectrometry (CoIP-MS), yeast two hybrid (Y2H) analysis, and computational predictions. We performed docking of ASP with its well-known receptor melanocortin receptor 4 (MC4R) to predict the binding capacity and to screen out actual ASP interacting proteins, CoIP-MS was performed where identified 32 proteins that could bind with ASP and Y2H confirmed seven proteins binding with ASP directly. CoIP-MS and Y2H screening results including PPI prediction revealed that vitronectin (VIN), apolipoprotein Al (AP0A1), apolipoprotein B (APOB), and filamin B (FLNB) were the key interacting proteins of ASP. VTN, AP0A1, and APOB are functional proteins in lipid metabolism and various skin disorders, suggesting ASP may function in lipid metabolism through these partners. This study provided protein-protein interaction information of ASP, and the results will promote further research into the diverse roles of ASP, as well as its binding partners, and their function in different strains of P. sinensis. (C) 2019 Published by Elsevier B.V.

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Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics