Danielsen, Mathias B.; Christensen, Niels Johan; Joergensen, Per T.; Jensen, Knud J.; Wengel, Jesper; Lou, Chenguang published an article in 2021. The article was titled 《Polyamine-Functionalized 2′-Amino-LNA in Oligonucleotides: Facile Synthesis of New Monomers and High-Affinity Binding towards ssDNA and dsDNA》, and you may find the article in Chemistry – A European Journal.Recommanded Product: Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine The information in the text is summarized as follows:
Attachment of cationic moieties to oligonucleotides (ONs) promises not only to increase the binding affinity of antisense ONs by reducing charge repulsion between the two neg. charged strands of a duplex, but also to augment their in vivo stability against nucleases. In this study, polyamine functionality was introduced into ONs by means of 2′-amino-LNA scaffolds. The resulting ONs exhibited efficient binding towards ssDNA, ssRNA and dsDNA targets, and the 2′-amino-LNA analog carrying a tri-aminated linker showed the most pronounced duplex- and triplex-stabilizing effect. Mol. modeling revealed that favorable conformational and electrostatic effects led to salt-bridge formation between pos. charged polyamine moieties and the Watson-Hoogsteen groove of the dsDNA targets, resulting in the observed triplex stabilization. All the investigated monomers showed increased resistance against 3′-nucleolytic digestion relative to the non-functionalized controls. The experimental process involved the reaction of Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine(cas: 510758-28-8Recommanded Product: Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine)
Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine(cas: 510758-28-8) can stabilizes Cu(I) towards disproportionation and oxidation thus enhancing its catalytic effect in the azide-acetylene cycloaddition.Recommanded Product: Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics