Rivera, Sylvia L.; Espaillat, Akbar; Aditham, Arjun K.; Shieh, Peyton; Muriel-Mundo, Chris; Kim, Justin; Cava, Felipe; Siegrist, M. Sloan published their research in Cell Chemical Biology in 2021. The article was titled 《Chemically Induced Cell Wall Stapling in Bacteria》.Category: triazoles The article contains the following contents:
Transpeptidation reinforces the structure of cell-wall peptidoglycan, an extracellular heteropolymer that protects bacteria from osmotic lysis. The clin. success of transpeptidase-inhibiting β-lactam antibiotics illustrates the essentiality of these crosslinkages for cell-wall integrity, but the presence of multiple, seemingly redundant transpeptidases in many species makes it challenging to determine crosslink function. Here, we present a technique to link peptide strands by chem. rather than enzymic reaction. We employ biocompatible click chem. to induce triazole formation between azido- and alkynyl-D-alanine residues that are metabolically installed in the peptidoglycan of Gram-pos. or Gram-neg. bacteria. Synthetic triazole crosslinks can be visualized using azidocoumarin-D-alanine, an amino acid derivative that undergoes fluorescent enhancement upon reaction with terminal alkynes. Cell-wall stapling protects Escherichia coli from treatment with the broad-spectrum β-lactams ampicillin and carbenicillin. Chem. control of cell-wall structure in live bacteria can provide functional insights that are orthogonal to those obtained by genetics. In the part of experimental materials, we found many familiar compounds, such as Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine(cas: 510758-28-8Category: triazoles)
Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine(cas: 510758-28-8) can stabilizes Cu(I) towards disproportionation and oxidation thus enhancing its catalytic effect in the azide-acetylene cycloaddition.Category: triazolesPolytriazolylamines were synthesized by the copper(I)-catalyzed ligation of azides and alkynes.
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics