Synthetic Route of C30H30N10In 2020 ,《Developing substrate-based small molecule fluorescent probes for super-resolution fluorescent imaging of various membrane transporters》 was published in Nanoscale Horizons. The article was written by Wu, Qiang; Cai, Mingjun; Gao, Jing; Zhao, Tan; Xu, Haijiao; Yan, Qiuyan; Jing, Yingying; Shi, Yan; Kang, Chuanqing; Liu, Yi; Liang, Feng; Chen, Junling; Wang, Hongda. The article contains the following contents:
Super-resolution imaging technol. has been a powerful tool for revealing fine biol. structures and functions. Its high-quality imaging always needs highly accurate labeling. Here, by exploiting the high specificity and affinity of natural substrates to transporters, we developed one set of substrate-based small mol. fluorescent probes for labeling membrane transporters. A glucose-based probe (Glu-probe) and tyrosine-based probe (Tyr-probe) were synthesized as two examples. Confocal imaging showed that the Glu-probe could label glucose transporters on live cells by being stuck into the binding site. Compared with antibody-probe labeling, the labeling advantages of the Glu-probe were revealed. High specificity of the Glu-probe or Tyr-probe was examined by a colocalization experiment and glucose replacement or amino acid (AA) blocking. The synthetic probes were also tested on imaging HeLa cells to confirm their wide labeling application. Addnl., we found that membrane transporters were mostly in the clustered state on cellular membranes, changing their assembly pattern to regulate the transport effectiveness. These results suggest that the substrate-based probes can serve as valuable tools for investigating the spatial information of membrane transporters.Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine(cas: 510758-28-8Synthetic Route of C30H30N10) was used in this study.
Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine(cas: 510758-28-8) is a polytriazolylamine ligand which stabilizes Cu(I) towards disproportionation and oxidation thus enhancing its catalytic effect in the azide-acetylene cycloaddition.Synthetic Route of C30H30N10
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics