An article Development of a 96-well based assay for kinetic determination of catalase enzymatic-activity in biological samples WOS:000580519700021 published article about INDUCED OXIDATIVE STRESS; HYDROGEN-PEROXIDE; SUPEROXIDE-DISMUTASE; C-ABL; CELLS; ANTIOXIDANT; EXPRESSION; GENE; DOXORUBICIN; GLUTATHIONE in [Grilo, Luis F.; Martins, Joao D.; Cavallaro, Chiara H.; Oliveira, Paulo J.; Pereira, Susana P.] CNC Ctr Neurosci & Cell Biol, UC Biotech Bldg,Biocant Pk, Cantanhede, Portugal; [Nathanielsz, Peter W.] Coll Agr & Nat Resources, Dept Anim Sci, Laramie, WY USA; [Pereira, Susana P.] Univ Porto, Fac Sport, Res Ctr Phys Act Hlth & Leisure CIAFEL, Porto, Portugal in 2020.0, Cited 64.0. Name: 1H-1,2,4-Triazol-5-amine. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5
Oxidative stress biomarkers are powerful endpoints in toxicological research. Cellular reductive/oxidative balance affects numerous signaling pathways involving H2O2. Detoxification and control of H2O2 levels results mainly from catalase activity. The aim of this work was to develop a precise, simple, cost-effective microassay to measure catalase activity in small tissue samples and cell extracts. We developed a protocol that quantifies H2O2 decomposition by intrinsic catalase in biological samples. Catalase activity was calculated based on rate of decomposition of H2O2, following absorbance at 240 nm. We developed a multi-well spectroscopic approach, reducing sample quantity requirements and allowing simultaneous assessment of large number of samples. The protocol is sensitive across a wide range of catalase activity (11.5-7575 U). The assay presents a 95% confidence interval with an intra-assay coefficient of variation of 3.7%, an inter-assay coefficient of variation of 6.2% and good correlation with a commercial kit. The assay was established and validated for different biological samples, including sheep hepatic tissue and human tumor and non-tumor cell lines. This high-throughput method is robust, sensitive, time-saving and cost-effective, generating highly reproducible results with precision and good correlation with a commercial kit reinforcing the method’s validity for research and toxicological applications.
Welcome to talk about 61-82-5, If you have any questions, you can contact Grilo, LF; Martins, JD; Cavallaro, CH; Nathanielsz, PW; Oliveira, PJ; Pereira, SP or send Email.. Name: 1H-1,2,4-Triazol-5-amine
Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
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