I found the field of Biotechnology & Applied Microbiology; Plant Sciences very interesting. Saw the article The AP2 transcription factor NtERF172 confers drought resistance by modifying NtCAT published in 2020.0. Name: 1H-1,2,4-Triazol-5-amine, Reprint Addresses Zhao, Q (corresponding author), Qingdao Agr Univ, Coll Hort, Qingdao, Peoples R China.; Li, YY (corresponding author), Hunan Tobacco Res Inst, Changsha, Hunan, Peoples R China.; Xiang, XH (corresponding author), Haikou Cigar Res Inst, Haikou, Hainan, Peoples R China.. The CAS is 61-82-5. Through research, I have a further understanding and discovery of 1H-1,2,4-Triazol-5-amine
Drought stress often limits plant growth and global crop yields. Catalase (CAT)-mediated hydrogen peroxide (H2O2) scavenging plays an important role in the adaptation of plant stress responses, but the transcriptional regulation of the CAT gene in response to drought stress is not well understood. Here, we isolated an APETALA2/ETHYLENE-RESPONSIVE FACTOR (AP2/ERF) domain-containing transcription factor (TF), NtERF172, which was strongly induced by drought, abscisic acid (ABA) and H2O2, from tobacco (Nicotiana tabacum) by yeast one-hybrid screening. NtERF172 localized to the nucleus and acted as a transcriptional activator. Chromatin immunoprecipitation, yeast one-hybrid assays, electrophoretic mobility shift assays and transient expression analysis assays showed that NtERF172 directly bound to the promoter region of the NtCAT gene and positively regulated its expression. Transgenic plants overexpressing NtERF172 displayed enhanced tolerance to drought stress, whereas suppression of NtERF172 decreased drought tolerance. Under drought stress conditions, the NtERF172-overexpressed lines showed higher catalase activity and lower accumulation of H2O2 compared with wild-type (WT) plants, while the NtERF172-silenced plants showed the inverse correlation. Exogenous application of amino-1,2,4-triazole (3-AT), an irreversible CAT inhibitor, to the NtERF172-overexpression lines showed decreased catalase activity and drought tolerance, and increased levels of cellular H2O2. Knockdown of NtCAT in the NtERF172-overexpression lines displayed a more drought stress-sensitive phenotype than NtERF172-overexpression lines. We propose that NtERF172 acts as a positive factor in drought stress tolerance, at least in part through the regulation of CAT-mediated H2O2 homeostasis.
Welcome to talk about 61-82-5, If you have any questions, you can contact Zhao, Q; Hu, RS; Liu, D; Liu, X; Wang, J; Xiang, XH; Li, YY or send Email.. Name: 1H-1,2,4-Triazol-5-amine
Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
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