Extracurricular laboratory: Synthetic route of 86404-63-9

I hope my short article helps more people learn about this compound(1-(2,4-Difluorophenyl)-2-(1H-1,2,4-triazol-1-yl)ethanone)Electric Literature of C10H7F2N3O. Apart from the compound(86404-63-9), you can read my other articles to know other related compounds.

In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called Method development and validation study for quantitative determination of genotoxic impurity and its precursor in fluconazole sample by liquid chromatography-tandem mass spectrometry, published in 2016, which mentions a compound: 86404-63-9, mainly applied to fluconazole liquid chromatog tandem mass spectrometry, Electric Literature of C10H7F2N3O.

Objective: The objective of this work is method development and validation study for quant. determination of 1-[2-(2,4-difluorophenyl)-2,3-epoxypropyl]-1H-1,2,4-triazole, a genotoxic impurity and its precursor in a fluconazole drug sample by liquid chromatog.-tandem mass spectrometry. Methods: LC-MS/MS anal. of these impurities was performed on Hypersil BDS C18 (100 mm x 4.0 mm, 3 μm) column. 5 mmol ammonium acetate and acetonitrile in the ratio of 65:35 (volume/volume) was used as the mobile phase with a flow rate of 0.4 mL/min. The developed method was accomplished with a short run time of 10 min. Triple quadrupole mass detector coupled with pos. electrospray ionization was used for the quantification of genotoxic impurities in multiple reaction monitoring (MRM). Results: The method was validated as per International Conference on Harmonization (ICH) guidelines. The method was linear in the range of 0.30 μg/g to 11.37 μg/g for impurity A and 0.30 μg/g to 11.34 μg/g for impurity B with a correlation coefficient of 0.999. The accuracy of the method was in the range of 98.25 % to 100.53 % for both impurities. Conclusion: A specific, selective, highly sensitive and more accurate anal. method using LC-MS/MS coupled with pos. electrospray ionization has been developed for the quantification of genotoxic impurity (1-[2-(2,4-difluorophenyl)-2,3-epoxypropyl]-1H-1,2,4-triazole) and its precursor (1-(2,4-difluorophenyl)-2-[1,2,4]triazol-1-yl-ethanone) at 0.3 μg/g with respect to the 5.0 mg/mL of fluconazole.

I hope my short article helps more people learn about this compound(1-(2,4-Difluorophenyl)-2-(1H-1,2,4-triazol-1-yl)ethanone)Electric Literature of C10H7F2N3O. Apart from the compound(86404-63-9), you can read my other articles to know other related compounds.

Reference:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics