In 2019,Physical Chemistry Chemical Physics included an article by Giannoulis, Angeliki; Yang, Yin; Gong, Yan-Jun; Tan, Xiaoli; Feintuch, Akiva; Carmieli, Raanan; Bahrenberg, Thorsten; Liu, Yangping; Su, Xun-Cheng; Goldfarb, Daniella. Formula: C12H22F6N6OP2. The article was titled 《DEER distance measurements on trityl/trityl and Gd(III)/trityl labelled proteins》. The information in the text is summarized as follows:
Triarylmethyl (TAM or trityl) radicals are becoming important for measuring distances in proteins and nucleic acids. Here, we report on a new trityl spin label CT02MA, which conjugates to a protein via a redox stable thioether bond. The performance of the new spin label was demonstrated in W-band double electron-electron resonance (DEER) distance measurements on doubly trityl-labeled mutants of IgG-binding protein 1 (GB1) and ubiquitin. For both doubly CT02MA-labeled proteins we measured, by applying chirped pump pulse(s), relatively narrow distance distributions, comparable to those obtained with the same protein mutants doubly labeled with BrPy-DO3MA-Gd(III). We noticed, however, that the sample contained some free CT02MA that was difficult to remove at the purification step. Dual labeling of ubiquitin with one CT02MA tag and one BrPy-DO3MA-Gd(III) tag was achieved as well and the trityl-Gd(III) distance distribution was measured, facilitated by the use of a dual mode cavity in combination with a chirped pump pulse. We also measured the Gd(III)-Gd(III) distance distribution in this sample, showing that the labeling procedure was not fully selective. Nevertheless, these measurements demonstrate the potential of the high sensitivity Gd(III)-trityl W-band DEER distance measurements in proteins, which can be further exploited by designing orthogonal Gd(III)/trityl labeling schemes. In the part of experimental materials, we found many familiar compounds, such as ((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V)(cas: 56602-33-6Formula: C12H22F6N6OP2)
((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V)(cas: 56602-33-6) is a peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.Formula: C12H22F6N6OP2
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics