Kaltenbach, Linda S. published the artcileComposite primary neuronal high-content screening assay for Huntington’s disease incorporating non-cell-autonomous interactions, Related Products of triazoles, the publication is Journal of Biomolecular Screening (2010), 15(7), 806-819, database is CAplus and MEDLINE.
Huntington’s disease (HD) is a fatal neurodegenerative disease characterized by progressive cognitive, behavioral, and motor deficits and caused by expansion of a polyglutamine repeat in the Huntingtin protein (Htt). Despite its monogenic nature, HD pathogenesis includes obligatory non-cell-autonomous pathways involving both the cortex and the striatum, and therefore effective recapitulation of relevant HD disease pathways in cell lines and primary neuronal monocultures is intrinsically limited. To address this, the authors developed an automated high-content imaging screen in high-d. primary cultures of cortical and striatal neurons together with supporting glial cells. Cortical and striatal neurons are transfected sep. with different fluorescent protein markers such that image-based high-content anal. can be used to assay these neuronal populations sep. but still supporting their intercellular interactions, including abundant synaptic interconnectivity. This assay was reduced to practice using transfection of a mutant N-terminal Htt domain and validated via a screen of ∼400 selected small mols. Both expected as well as novel candidate targets for HD emerged from this screen; of particular interest were target classes with close relative proximity to clin. testing. These findings suggest that composite primary cultures incorporating increased levels of biol. complexity can be used for high-content imaging and “high-context” screening to represent mol. targets that otherwise may be operant only in the complex tissue environment found in vivo during disease pathogenesis.
Journal of Biomolecular Screening published new progress about 377727-87-2. 377727-87-2 belongs to triazoles, auxiliary class GPCR/G Protein,Adenosine Receptor, name is 2-(Furan-2-yl)-7-(2-(4-(4-(2-methoxyethoxy)phenyl)piperazin-1-yl)ethyl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine, and the molecular formula is C25H29N9O3, Related Products of triazoles.
Referemce:
https://en.wikipedia.org/wiki/1,2,3-Triazole,
Triazoles – an overview | ScienceDirect Topics