Structural Characterization of Serum N-Glycans by Methylamidation, Fluorescent Labeling, and Analysis by Microchip Electrophoresis was written by Mitra, Indranil;Snyder, Christa M.;Zhou, Xiaomei;Campos, Margit I.;Alley, William R.;Novotny, Milos V.;Jacobson, Stephen C.. And the article was included in Analytical Chemistry (Washington, DC, United States) in 2016.Related Products of 156311-83-0 The following contents are mentioned in the article:
To characterize the structures of N-glycans derived from human serum, the authors report a strategy that combines microchip electrophoresis, standard addition, enzymic digestion, and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The authors compared: (1) electrophoretic mobilities of known N-glycans from well-characterized (standard) glycoproteins through standard addition, (2) the electrophoretic mobilities of N-glycans with their mol. weights determined by MALDI-MS, and (3) electrophoretic profiles of N-glycans enzymically treated with fucosidase. The key step to identify the sialylated N-glycans was to quant. neutralize the neg. charge on both α2,3- and α2,6-linked sialic acids by covalent derivatization with methylamine. Both neutralized and nonsialylated N-glycans from these samples were then reacted with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) to provide a fluorescent label and a triple-neg. charge, separated by microchip electrophoresis, and detected by laser-induced fluorescence. The methylamidation step leads to a 24% increase in the peak capacity of the separation and direct correlation of electrophoretic and MALDI-MS results. In total, 37 unique N-glycan structures were assigned to 52 different peaks recorded in the electropherograms of the serum samples. This strategy ensures the needed separation efficiency and detectability, easily resolves linkage and positional glycan isomers, and is highly reproducible. This study involved multiple reactions and reactants, such as ((3H-[1,2,3]Triazolo[4,5-b]pyridin-3-yl)oxy)tri(pyrrolidin-1-yl)phosphonium hexafluorophosphate(V) (cas: 156311-83-0Related Products of 156311-83-0).
((3H-[1,2,3]Triazolo[4,5-b]pyridin-3-yl)oxy)tri(pyrrolidin-1-yl)phosphonium hexafluorophosphate(V) (cas: 156311-83-0) belongs to triazole derivatives. However, triazoles are also useful in bioorthogonal chemistry, because the large number of nitrogen atoms causes triazoles to react similar to azides. Many triazoles have antifungal effects: the triazole antifungal drugs include fluconazole, isavuconazole, itraconazole, voriconazole, pramiconazole, ravuconazole, and posaconazole and triazole plant-protection fungicides include epoxiconazole, triadimenol, myclobutanil, propiconazole, prothioconazole, metconazole, cyproconazole, tebuconazole, flusilazole and paclobutrazol.Related Products of 156311-83-0
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics