Palacharla, Raghava Choudary et al. published their research in Xenobiotica in 2019 | CAS: 1614-12-6

1H-Benzo[d][1,2,3]triazol-1-amine (cas: 1614-12-6) belongs to triazole derivatives. Triazoles consist of a five-membered ring containing three nitrogen atoms and are biologically active, especially as antifungal, antimicrobial and enzyme inhibitors. 1,2,3-Triazoles are usually prepared following (3+2) cycloaddition protocols. A common technique for unsubstituted triazoles is the Huisgen azide-alkyne 1,3-dipolar cycloaddition: a azide and an alkyne react at high temperature to form a ring. However, the Huisgen strategy produces a mixture of isomers (typically 1,4- and 1,5-disubstituted) when used to produce substituted triazoles.Category: triazoles

Methoxsalen as an in vitro phenotyping tool in comparison with 1-aminobenzotriazole was written by Palacharla, Raghava Choudary;Molgara, Parusharamulu;Panthangi, Hanumanth Rao;Boggavarapu, Rajesh Kumar;Manoharan, Arun Kumar;Ponnamaneni, Ranjith Kumar;Ajjala, Devender Reddy;Nirogi, Ramakrishna. And the article was included in Xenobiotica in 2019.Category: triazoles This article mentions the following:

The objective is to evaluate methoxsalen as an in vitro phenotyping tool in comparison to ABT as a nonspecific inactivator of P 450 mediated metabolism The reversible inhibition of methoxsalen and ABT against the P 450, FMO, AO, MAO-A and -B, enzymes were evaluated using standard marker probe reactions. The time-dependent inhibition of P 450 enzymes was evaluated in human liver microsomes. CES1 activities were determined by monitoring the depletion of known substrate, the clopidogrel. The metabolism of P 450 substrates in the presence and absence of methoxsalen or ABT was evaluated in human liver microsomes. Methoxsalen is a direct inhibitor and inhibited the activities (>90%) of all enzymes at a concentration of 300μM except for CYP2C9. Methoxsalen is also a potent time-dependent inhibitor of all P 450 enzymes except for CYP2C19 (moderate) at a concentration of 300μM. Methoxsalen inhibited the metabolism of P 450 substrates in the pre-incubation mode. ABT is a potent TDI of several P 450 except for CYP2C19 (47%) and CYP2C9 (27%). The results indicate that methoxsalen is a potent pan P 450 inhibitor than ABT and can be a better tool in distinguishing P 450 mediated metabolism form non-P 450 metabolism in human liver microsomes. In the experiment, the researchers used many compounds, for example, 1H-Benzo[d][1,2,3]triazol-1-amine (cas: 1614-12-6Category: triazoles).

1H-Benzo[d][1,2,3]triazol-1-amine (cas: 1614-12-6) belongs to triazole derivatives. Triazoles consist of a five-membered ring containing three nitrogen atoms and are biologically active, especially as antifungal, antimicrobial and enzyme inhibitors. 1,2,3-Triazoles are usually prepared following (3+2) cycloaddition protocols. A common technique for unsubstituted triazoles is the Huisgen azide-alkyne 1,3-dipolar cycloaddition: a azide and an alkyne react at high temperature to form a ring. However, the Huisgen strategy produces a mixture of isomers (typically 1,4- and 1,5-disubstituted) when used to produce substituted triazoles.Category: triazoles

Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics