Quality Control of ((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V)In 2015 ,《A Novel Bio-Orthogonal Cross-Linker for Improved Protein/Protein Interaction Analysis》 appeared in Analytical Chemistry (Washington, DC, United States). The author of the article were Nury, Catherine; Redeker, Virginie; Dautrey, Sebastien; Romieu, Anthony; van der Rest, Guillaume; Renard, Pierre-Yves; Melki, Ronald; Chamot-Rooke, Julia. The article conveys some information:
The variety of protein crosslinkers developed in recent years illustrates the current requirement for efficient reagents optimized for mass spectrometry (MS) anal. To date, the most widely used strategy relies on com. crosslinkers that bear an isotopically labeled tag and N-hydroxysuccinimid-ester (NHS-ester) moieties. Moreover, an enrichment step using liquid chromatog. is usually performed after enzymic digestion of the crosslinked proteins. Unfortunately, this approach suffers from several limitations. First, it requires large amounts of proteins. Second, NHS-ester crosslinkers are poorly efficient because of their fast hydrolysis in water. Finally, data anal. is complicated because of uneven fragmentation of complex isotopic crosslinked peptide mixtures We therefore synthesized a new type of trifunctional crosslinker to overrule these limitations. This reagent, named NNP9, comprises a rigid core and bears two activated carbamate moieties and an azido group. NNP9 was used to establish intra- and intermol. crosslinks within creatine kinase, then to map the interaction surfaces between α-Synuclein (α-Syn), the aggregation of which leads to Parkinson’s disease, and the mol. chaperone Hsc70. We show that NNP9 crosslinking efficiency is significantly higher than that of NHS-ester com. crosslinkers. The number of crosslinked peptides identified was increased, and a high quality of MS/MS spectra leading to high sequence coverage was observed Our data demonstrate the potential of NNP9 for an efficient and straightforward characterization of protein-protein interfaces and illustrate the power of using different crosslinkers to map thoroughly the surface interfaces within protein complexes. After reading the article, we found that the author used ((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V)(cas: 56602-33-6Quality Control of ((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V))
((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V)(cas: 56602-33-6) is used as a reagent for peptide coupling, lactonization, selective esterification, amidation of alfa amino acids without racemization and synthesis of magnolamide for antioxidative activity and catalyst for 9-acridinecaroboxamide derivative. It is also used as a precursor for the synthesis of phenyl esters of amino acids.Quality Control of ((1H-Benzo[d][1,2,3]triazol-1-yl)oxy)tris(dimethylamino)phosphonium hexafluorophosphate(V)
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics