In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called A validated stability-indicating liquid chromatographic method for determination of degradation impurities and diastereomers in voriconazole tablets, published in 2012-12-31, which mentions a compound: 86404-63-9, mainly applied to voriconazole determination RP HPLC diastereomer degradation impurity tablet; Development; Forced Degradation study; HPLC; Validation; Voriconazole, Reference of 1-(2,4-Difluorophenyl)-2-(1H-1,2,4-triazol-1-yl)ethanone.
A reversed-phase gradient liquid chromatog. method was developed for the quant. determination of Voriconazole, along with its degradation and diastereomeric impurities in tablet dosage form. Chromatog. separation was achieved on an Inertsil ODS 3V, 150 × 4.6 mm, 5 μm column. The mobile phase consisting of solvent A 0.05 M (M) potassium dihydrogen phosphate (pH 2.5 buffer) and solvent B (mixture of acetonitrile and MeOH in the ratio 90:10 (volume/volume)), was delivered at a flow rate of 1.2 mL min-1 with the detection wavelength at 256 nm. Resolution of Voriconazole and all 5 potential impurities was achieved at > 2.0 for all pairs of compounds The drug was subjected to stress conditions such as oxidative, acid and base hydrolysis, and thermal and photolytic degradation Voriconazole was found to degrade significantly under base hydrolysis stress conditions compared to acid hydrolysis stress conditions. The degradation products were well-resolved from the main peak and its impurities, thus proving the stability-indicating power of the method. The stressed samples were assayed against a reference standard and the mass balance was found to be close to 99.0%. The developed method was validated as per ICH guidelines with respect to specificity, linearity, limit of detection, limit of quantification, accuracy, precision, and robustness.
Here is a brief introduction to this compound(86404-63-9)Reference of 1-(2,4-Difluorophenyl)-2-(1H-1,2,4-triazol-1-yl)ethanone, if you want to know about other compounds related to this compound(86404-63-9), you can read my other articles.
Reference:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics