In 2018,Angewandte Chemie, International Edition included an article by Wagner, Nicolai; Stephan, Milena; Hoeglinger, Doris; Nadler, Andre. Electric Literature of C30H30N10. The article was titled 《A Click Cage: Organelle-Specific Uncaging of Lipid Messengers》. The information in the text is summarized as follows:
Lipid messengers exert their function on short time scales at distinct subcellular locations, yet most exptl. approaches for perturbing their levels trigger cell-wide concentration changes. Herein, we report on a coumarin-based photocaging group that can be modified with organelle-targeting moieties by click chem. and thus enables photorelease of lipid messengers in distinct organelles. We show that caged arachidonic acid and sphingosine derivatives can be selectively delivered to mitochondria, the ER, lysosomes, and the plasma membrane. By comparing the cellular calcium transients induced by localized uncaging of arachidonic acid and sphingosine, we show that the precise intracellular localization of the released second messenger is crucial for the signaling outcome. Ultimately, we anticipate that this new class of caged compounds will greatly facilitate the study of cellular processes on the organelle level. The experimental part of the paper was very detailed, including the reaction process of Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine(cas: 510758-28-8Electric Literature of C30H30N10)
Tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine(cas: 510758-28-8) is a polytriazolylamine ligand which stabilizes Cu(I) towards disproportionation and oxidation thus enhancing its catalytic effect in the azide-acetylene cycloaddition.Electric Literature of C30H30N10
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics