Category: Triazoles. Andrews, SS; Schaefer-Ramadan, S; Al-Thani, NM; Ahmed, I; Mohamoud, YA; Malek, JA in [Andrews, Simeon S.; Schaefer-Ramadan, Stephanie; Al-Thani, Nayra M.; Ahmed, Ikhlak; Malek, Joel A.] Weill Cornell Med Qatar, Dept Genet Med, Doha 24144, Qatar; [Mohamoud, Yasmin A.; Malek, Joel A.] Weill Cornell Med Qatar, Genom Lab, Doha 24144, Qatar published High-resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-Seq) in 2019.0, Cited 18.0. The Name is 1H-1,2,4-Triazol-5-amine. Through research, I have a further understanding and discovery of 61-82-5.
Two-hybrid systems can be used for investigating protein-protein interactions and may provide important information about gene products with unknown function. Despite their success in mapping protein interactions, two-hybrid systems have remained mostly untouched by improvements in next-generation DNA sequencing. The two-hybrid systems rely on one-versus-all methods in which each bait is sequentially screened against an entire library. Here, we developed a screening method that joins both bait and prey as a convergent fusion into one bacterial plasmid vector that can then be amplified and paired-end sequencing by next-generation sequencing (NGS). Our method enables all-versus-all sequencing (AVA-Seq) and utilizes NGS to remove multiple bottlenecks of the two-hybrid system. AVA-Seq allows for high-resolution protein-protein interaction mapping of a small set of proteins and has the potential for lower-resolution mapping of entire proteomes. Features of the system include ORF selection to improve efficiency, high bacterial transformation efficiency, a convergent fusion vector to allow paired-end sequencing of interactors, and the use of protein fragments rather than full-length proteins to better resolve specific protein contact points. We demonstrate the system’s strengths and limitations on a set of proteins known to interact in humans and provide a framework for future large-scale projects.
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Reference:
Article; Safari, Niloufar; Shirini, Farhad; Tajik, Hassan; Journal of Molecular Structure; vol. 1201; (2020);,
1,2,3-Triazole – Wikipedia,
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