Proteomic analysis of liver proteins of mice exposed to 1,2-dichloropropane was written by Zhang, Xiao;Morikawa, Kota;Mori, Yurie;Zong, Cai;Zhang, Lingyi;Garner, Edwin;Huang, Chinyen;Wu, Wenting;Chang, Jie;Nagashima, Daichi;Sakurai, Toshihiro;Ichihara, Sahoko;Oikawa, Shinji;Ichihara, Gaku. And the article was included in Archives of Toxicology in 2020.Product Details of 1614-12-6 This article mentions the following:
The aim of the present study was to characterize the mol. mechanisms of 1,2-DCP-induced hepatotoxic effects by proteomic anal. We analyzed quant. the differential expression of proteins in the mouse liver and investigated the role of P 450 in mediating the effects of 1,2-DCP. Male C57BL/6JJcl mice were exposed to 0, 50, 250, or 1250 ppm 1,2-DCP and treated with either 1-aminobenzotriazole (1-ABT), a nonselective P 450 inhibitor, or saline, for 8 h/day for 4 wk. Two-dimensional difference in gel electrophoresis (2D-DIGE) combined with matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF/MS) was used to detect and identify proteins affected by the treatment. PANTHER overrepresentation test on the identified proteins was conducted. 2D-DIGE detected 61 spots with significantly different intensity between 0 and 250 ppm 1,2-DCP groups. Linear regression anal. showed significant trend with 1,2-DCP level in 17 proteins in mice co-treated with 1-ABT. 1-ABT mitigated the differential expression of these proteins. The gene ontol. enrichment anal. showed overrepresentation of proteins functionally related to nickel cation binding, carboxylic ester hydrolase activity, and catalytic activity. The results demonstrated that exposure to 1,2-DCP altered the expression of proteins related with catalytic and carboxylic ester hydrolase activities, and that such effect was mediated by P 450 enzymic activity. In the experiment, the researchers used many compounds, for example, 1H-Benzo[d][1,2,3]triazol-1-amine (cas: 1614-12-6Product Details of 1614-12-6).
1H-Benzo[d][1,2,3]triazol-1-amine (cas: 1614-12-6) belongs to triazole derivatives. Triazoles exhibit substantial isomerism, depending on the positioning of the nitrogen atoms within the ring. Triazoles are compounds with a vast spectrum of applications, varying from materials (polymers), agricultural chemicals, pharmaceuticals, photoactive chemicals and dyes.Product Details of 1614-12-6
Referemce:
1,2,3-Triazole – Wikipedia,
Triazoles – an overview | ScienceDirect Topics